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Nupur Agarwal Amity Institute Of Food Technology, Noida, Uttar Pradesh ,Sector 125,India
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mFFm T OF PH mnzymes are affected by changes in pH. The most favorable pH value - the point where the enzyme is most active - is known as the optimum pH. mtremely high or low pH values generally result in complete loss of activity for most enzymes.
=ike most chemical reactions, the rate of an enzyme-catalyzed reaction increases as the temperature is raised. A ten degree entigrade rise in temperature will increase the activity of most enzymes by 50 to 100%. Most animal enzymes rapidly become denatured at temperatures above 40°
If the amount of the enzyme is kept constant and the substrate concentration is then gradually increased, the reaction velocity will increase until it reaches a maimum. After this point, increases in substrate concentration will not increase the velocity
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=ASSIFI ATION OF mNZYMmS ON THm BASIS OF HmMI A= RmA TIONS ATA=ISm
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Polygalacturonase
tomato
1.00
Pineapple
0.024
carrot
o
grape
o
soybeans
1.00
peas
0.35
wheat
0.02
peanuts
0.01
Green peas
1.00
spinach
0.32
Lipoxygenase
Peroxidase
RO=m OF mNZYMmS IN FOO PRO mSSING
mnzymes used as processing aids And ingredients mNZYMmS USm IN STAR H INSTRY 1.Production of high fructose corn syrup and sweeteners. Úmm
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GLw
Lw GL Lw
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Lw GL Lw GL w w
starch · detrins
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isomerase fructose glucose
mNZYMmS USm IN MI= AN AIRY PROU TS m m
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wame as above
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mNZYMmS IN BA ING m m
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mNZYMmS IN BRmING m m
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RO=m OF mNZYMmS IN FOO QA=ITY Quality has been defined as degree of ecellance which includes colour,teture,flavor and aroma
Three key enzymesresponsible for chemical alterations of pigments in fruits and vegetables are m m
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Tetural factors include hand feel and mouth feel of firmness ,softness, juiciness, chewiness and grittiness . m m
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mnzymes cause off flavors and off aromas in foods, particularly during storage m m
Lipoxygenase
nglish green peas grean beans and corn
ystine lyase
Broccoli and cauliflower
naringin
Grapefruit its juice(bitter taste)
mnzymes do have a role to play in nutritional quality of food. m m
mÚÚm
Lipoxygenase
Decrease the amount of essential fatty acids in foods. Decrease carotenoids it it etc (due to free radicals produced during oxidation)
Polyphenol oxidase
Decrease available lysine content of proteins(browning)
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