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BIOSTAT ® B The Multi-Talented Bioreactor for Research and Development
BIOSTAT® B at a Glance Our BIOSTAT® B is the ideal benchtop bioreactor for your lab. The multi-talented control tower opens up a new world of flexibility for your changing requirements. Use it as single or twin configuration, chose your cultivation chamber from our proven range of options: – Conventional stirred tank glass vessels – Single-use stirred tank vessel Univessel® SU – Wave-mixed Flexsafe RM bags
Applications – Process development and optimization – Scale-up and scaledown studies – Seed expansion – Cell bank production – Protein supply
Cells – Mammalian – Insect – Microbial – Yeast – Fungi
Industries – Biopharmaceuticals – Vaccines – Cell therapies – Industrial biotechnology – Basic research – Education
Process Modes – Batch – Fed-batch – Continuous – Perfusion
Proven Technology With several thousand installations in over 50 countries, BIOSTAT® B is the most successful benchtop bioreactor in the world. Benefit from our experience and collaboration with customers worldwide.
Configurable Design your Sartorius representative and configure your BIOSTAT® B bioreactor solution matching your specific needs. Benefit from our flexible and scalable options.
Reliable Quality Every BIOSTAT® B is thoroughly tested before it leaves our production facilities in . Benefit from our global service and application specialist network for professional installation and training.
UniVessel® Glass Our proven autoclavable borosilicate glass culture vessel is available in four different volumes: 1 L, 2 L, 5 L and 10 L, for all kinds of cell culture and microbial applications.
Benefits −− Classic stirred-tank design simplifies your scale-up and scale-down studies −− Extensive performance and engineering data package available −− Save additional investment and use our special design for small autoclaves
Fully interchangable glass and single-use stirred-tank culture vessels.
UniVessel® SU Our single-use 2 L stirred tank vessel for cell culture applications combines scalable design with all benefits of disposable solutions. No more worries about challenging timelines, workload or cross-contamination. Get started within minutes.
Benefits −− Fully single-use vessel, DO and pH sensors −− Proven, scalable stirred tank design −− Connection kit to upgrade existing bioreactor controllers
BIOSTAT® B – The Universal Controller for Your Lab
Main Applications −− Mammalian, insect, microbial and plant cell culture −− Process development, optimization, characterization −− Seed and small scale production −− Protein supply
Main Applications −− Mammalian, insect and plant cell culture −− Process development, optimization, characterization −− Seed and small scale production −− Production of cell banks
Watch Video: www.sartorius.com/ video-biostat-b
Main Applications −− Mammalian, insect, plant and low density microbial culture −− Seed and small scale production −− Protein supply −− Stem cell expansion and production
RM Rocker Our proven wave-mixed bioreactor consists of a rocker unit with bag holder and pillow-shaped Flexsafe RM single-use bags for culture volumes from 100 mL to 25 L. Use it as basic system or in combination with the BIOSTAT® B control tower and single-use optical pH and DO sensors to enable advanced control strategies for batch, fed-batch and continuous perfusion.
Benefits −− Optimized film formula, traceability of raw materials and control of the entire Flexsafe RM bag manufacturing process assure excellent and consistent cell growth of the most sensitive cell lines −− Space-saving, individual control of two RM bags on the same rocker platform −− Easy-to-use rocker with advanced alarms and safety features for reliable cultivation
12” Touch Screen with closed frame; protected against water splashes and dust deposits. Easy-to-use and reliable operation of your BIOSTAT® B due to intuitive design of human-machine interface and advanced touchscreen technology – even while wearing laboratory gloves.
New Standards for Interference-Free Measurements All inlets and ports for, e.g. cooling water, process gasses, electricity, ethernet and potential-free alarm are located on the rear of the control tower. An equipotential bonding conductor shields the bioreactor against electromagnetic current and ensures interference-free measurements during your processes.
Control Tower The control tower contains both the aeration, pump and temperature control modules, saving valuable bench space in your lab.
BIOSTAT® B Configurable Flexibility Single | Twin Control Tower One BIOSTAT® B control tower can control up to two vessels completely independently to save valuable lab space. Select culture vessel types and volumes from our range of options, e.g. glass vessel, single-use vessel or wave-mixed Flexsafe® RM bags.
Fast Load Peristaltic Pumps Insert the tubing in seconds: Open, insert tube, close, done! No more pinched fingers or torn gloves. Up to four internal pumps can be selected per vessel. Choose between speedcontrolled pumps and fixedspeed pumps for feed and corrective agent addition.
Connections for Sensors and External Accessories Quick-connect couplings make it easy to attach all cables and supplies to the culture vessels. All connections are clearly marked with the same names used on the rear control tower and in the local control software menu for fast, error proof identification.
Storage Tray A storage tray for accessories helps you organize your workplace.
Aeration Strategies A series of flexible aeration options turns the BIOSTAT® B into a multitalented bioreactor for a wide range of different applications, including high-cell density microbial fermentation requiring considerable amounts of oxygen to high-cell density cell culture applications requiring removal of excessive carbon dioxide.
Microbial Applications in Combination with UniVessel® Glass Various controller and hardware configurations enable aeration strategies using air or oxygen or classical O2 enrichment of air. For anaerobic processes, the air inlet can be used for nitrogen. The standard built-in solenoid valves in combination with a flow meter ensure reliable gas supply for simple applications. Optional mass flow controllers provide exact flow rate control of individual gases. In combination with BioPAT® Xgas off-gas analysis, this allows mass balancing consumed and produced gases.
Gas Inlet
Solenoid Valve MFC* (max. 2) (max. 2)
Air
Flow Meter
Gas Outlet
Sparger
O2 *MFC = mass flow controller
Cell Culture and Multi-Purpose Applications in Combination with UniVessel® Glass and UniVessel® SU Gas Inlet
Solenoid Valve MFC * (4 max.) (4 max.)
Flow Meter
Gas Outlet
Overlay
Air
Sparger
O2 N2 CO2 Switching CO2 from sparger to overlay *MFC = mass flow controller
Five different gas lines, equipped with solenoid valves and|or up to four optional mass flow controllers and flow meters deliver ultimate flexibility and accuracy. The BIOSTAT® B allows you to optionally switch between sparger and overlay supply of CO2 for pH control. In addition, a constant air | CO2 mixture can be created in the overlay line to reproduce conditions like those prevailing in a CO2 incubator.
Cell Culture Applications in Combination with Our Rocking-Motion Bioreactor The wave generated in the RM bags due to the rocking motion ensures effective gas exchange through the gas-liquid interface. Four different gas lines for air, O2, N2 and CO2 are equipped with flow meters and four high-precision mass flow controllers. An integrated pressure sensor continuously measures the pressure inside the bag. To ensure process safety, aeration is switched off when an upper threshold is reached.
Gas Inlet
MFC*
Flow Meter
Gas Outlet
Overlay
Air O2 N2 CO2 *MFC = mass flow controller
You can configure your BIOSTAT® B aeration system in a similar way as your BIOSTAT® STR single-use stirred tank bioreactor. Get ready for seamless scale-up and -down, from laboratory scale to large scale single-use production.
DCU – Local Control Since we introduced the first DCU-controlled bioreactors back in the late 80’s, we have installed thousands of such bioreactors to date at leading companies in the pharmaceutical and biotech industry worldwide. We have continuously improved our robust, intuitive-use and industry-proven DCU control technology, now available in its fourth generation. It is our standard local control platform for our BIOSTAT® bioreactors, SARTOFLOW® crossflow filtration units and FlexAct® configurable systems available for a large number of unit operations. The intuitive touch screen on the control tower is used to locally operate the BIOSTAT® B: Aeration control
Balances and gravimetric flow control
Overview of process parameters
Pump module
Submenus
Overview and close-up Culture vessels
Status display
Automatic Feed Control and Continuous Processing Design your process strategy or select different options. Configure your BIOSTAT® B with gravimetric feed control, gravimetric level control or substrate addition profiles. This enables you to run your BIOSTAT® B in batch, fed-batch, continuous or perfusion mode.
Automatic DO Control Besides classic DO cascade control, we have developed the unique advanced DO controller that gives you more flexibility to develop and optimize your DO control strategy. The advanced DO controller s parallel adjustment of all DO affecting parameter settings like stirrer speed and gas flow rates of air and pure oxygen, automatically and simultaneously to control the DO set point. Optionally, you can keep the total gas flow rate constant and the ratio of pure oxygen in the gas mixture is increased automatically to match the oxygen consumption of the culture. This gives you ultimate flexibility to adapt your aeration strategy to your process requirements.
Automatic pH Control Control the pH of your culture by automatic acid and base addition or by CO2 aeration and base addition. If you want to use your BIOSTAT® B for both microbial and cell culture you can configure your bioreactor system with a combined acid control via CO2 aeration and acid addition.
CO2
Base Acid
BioPAT® MFCS – SCADA Process Control BioPAT® MFCS is a multi-functional supervisory control and data acquisition software, specifically developed for bioprocessing applications in upstream and downstream. Every BIOSTAT® bioreactor comes with basic data recording functionality of our BioPAT® MFCS. Rely on more than 7,000 installations worldwide and over 25 years of experience. – Multi- network access for up to 16 process units – On-line and off-line sample data management, visualization, evaluation – Remote monitoring, control and alarming
BioPAT® MFCS | win In addition to providing a genuine SCADA system for conventional and single-use bioprocess applications, the optional advanced version of BioPAT® MFCS includes a series of add-on modules: – Bioprocess optimization with Design of Experiments module (DoE) – Automation with recipes according to ANSI | ISA-88.01 – 21 CFR, Part 11 compatibility – Secure integration into company networks
– Flexible device connection facility through OPC – Expanded data recording and data archiving capabilities – Alarm recording and transmission to multimedia devices
Our Mission Is Your Productivity We are well aware of how important smooth-running equipment is for effective process development and efficient production. That is why we at Sartorius offer you a broad range of preventive technical service, repair and application specialist . Our ISO certified quality management system ensures that you receive equipment and services that meet the strictest quality requirements. Become one of our many customers who benefit from our local presence worldwide, highly qualified specialists and well-versed technicians.
Services Offered for BIOSTAT® B −− On-site installation and application specialist , ensuring that your equipment is perfectly up and unning right from the start −− Factory acceptance and site-acceptance testing −− On-site preventive maintenance and repair −− Consumable and spare part kits
Technical Specifications BIOSTAT® B Control Tower Weight Single | Twin
~ 40 | 55 kg (88 | 121 lbs.)
Control Tower Dimensions (W + H + D) Single | Twin
410 + 810 + 520 mm (16“ + 32“ + 20“)
Utility Connections Power supply
– 230 V (± 10%), 50 Hz, max. power consumption 10 A – 120 V (± 10%), 60 Hz, max. power consumption 12 A – Potential equalisation
International protection rating
IP21
Gases
– Gas supply pressure, 1.5 barg – Dry, oil and dust-free – Hose barb for tubing, external dia. = 6 mm
Water
– Water supply pressure, 2 – 8 barg – Flow rate up to 20 lpm – Temperature min. = 4°C – Discharge pressure-less – Hose barb for tubing, external dia. = 10 mm – Degree of hardness: 12 dH max.
Control Tower Housing
Stainless steel, AISI 304
Display
Touch screen, 12”, glass, capacitive
Resolution
125 dpi
SCADA communication
Industrial Ethernet
Potential-free (common) alarm
•
Safety valve gas pressure
1 bar (14.5 psi) for UniVessel® Glass and SU | 100 mbar for RM
Water inlet pressure reduction
1.5 bar (22 psi), integrated pressure control
®
Motor Drive (Univessel Glass | SU) Maintenance-free, quiet direct drive
Power: 200 W
Maintenance-free top drive with magnetic motor coupling
Power: 200 W
Rotation speed motor, direct coupling
1L: 20 – 2,000 rpm 2L: 20 – 2,000 rpm 5L: 20 – 1,500 rpm 10L: 20 – 800 rpm 2L SU: 20 – 400 rpm
Rocker Platform Power supply
– 230 V (± 10%), 50 Hz, power consumption 10 A – 120 V (± 10%), 60 Hz, power consumption 12 A
Power consumption
600 W
International protection rating
IP23
Rocking speed
8 – 42 ± 1 (r/min)
Rocking angle
4 –10 ± 0.3 (°)
Process Control | Sensors UniVessel® Glass
UniVessel® SU
Rocking Motion
Pt100 | 0–150°C (temperature control 0-80°C) | 0.1°C
•
•
•
Dissolved oxygen, reusable
Polarographic or optical | 0–100 % | 0.1 %
•
•
Dissolved oxygen, single-use
Range: 0 –100 % Precision: 0.1%
pH, reusable
Combined measuring electrode Range: 2–12 pH Precision: 0.01 pH
pH, single-use
Range: 6.5 - 8.5 pH Precision: 0.1 pH
Foam control
Electrical conductive sensor, stainless steel, ceramic insulated
•
Level
Electrical conductive sensor, stainless steel, ceramic insulated
•
Turbidity
1-channel NIR absorption sensor | 0–6 AU | 0,01 AU
•
Redox
Combined measurement with pH sensor | -1,000 – 1,000 mV | 1 mV
•
•
Balance substrate
7 kg max. | 1 g 60 kg max. | 2 g 300 kg max. | 100 g
•
•
Balance culture vessel
60 kg max. | 2 g
•
•
RM load cells
30 kg max. | 10 g Weight: 9 kg Dimensions: 609 | 536 | 60-68 mm 100–240 V VAC | 24 VDC | 15 W
External signal input
0–10 V or 4-20 mA UniVessel® Glass | SU: 4 max. Rocker: 2 max.
Sensor | Measurement Range | Display Accuracy Temperature
• •
•
• •
•
•
•
•
•
•
Aeration Module Outlet to culture vessel | bag ®
UniVessel Glass MO (Microbial)
Hose coupling d external = 6 mm Two-gas mixing with sparger outlet
AIR with O2 Enrichment or Gas Flow Ratio mixing along with anaerobic fermentation; for further information, please see page on “aeration strategies” Max. total flow
Up to 20 l/min total volume flow
Flow rate controllers
2
Flow rate controller range
Various models available: 0.1-20 liters per min. [lpm] (sparger)
Accuracy of flow rate controllers
± 5 % full scale
Mass flow controllers (optional)
2 max.
Flow rates of mass flow controllers
Various models available: 0.03-20 lpm (sparger)
Accuracy of mass flow controllers
± 1 % full scale
®
UniVessel Glass CC (Cell Culture) | UniVessel® SU
Four-gas mxing with sparger and headspace outlet
Additive flow, 4-gas (Air, O2, N2, CO2) mixture; for further information, please see page on “Aeration Strategies” Max. total flow
Up to 13 l/min total volume flow
Number of flow rate controllers
5
Flow rate controller range
Various models available: 3.3 ccm – 1.6 lpm (sparger) 0.16 lpm – 13 lpm (headspace)
Accuracy of flow rate controllers
± 5 % FS
Mass flow controllers (optional)
4 max.
Flow rates of mass flow controllers
Various models available: 1 ccm – 1.5 lpm (sparger) 0.03 lpm – 10 lpm (headspace)
Precision of mass flow controllers
± 1 % FS
UniVessel® Glass, Dual Use
Four-gas mixing with sparger and headspace outlet
Additive flow, 4-gas (Air, O2, N2, CO2) mixture; for further information, please see page on “Aeration Strategies” Max. total flow
Up to 20 l/min total volume flow
Flow rate controllers
5
Flow rate controller range
Various models available: 3.3 ccm – 20 lpm (sparger) 50 ccm – 20 lpm (headspace)
Accuracy of flow rate controllers
± 5% full scale
Mass flow controllers (optional)
4 max.
Flow rates of mass flow controllers
Various models available: 0.6 ccm – 20 lpm (sparger) 10 ccm – 20 lpm (headspace)
Precision of mass flow controllers
± 1 % FS
Rocker
Four-gas mixing with sparger and headspace outlet
Additive flow, 4-gas (Air, O2, N2, CO2) mixture; for further information, please see page on “Aeration Strategies” Max. total flow
Up to 10 l/min total volume flow
Max. CO2 flow
One bag: 1 + 1 lpm Twin bag: 2 + 0.5 lpm
CO2 concentration controller
0.8 % – 15% | ± 5%
Flow rate controllers
4
Flow rate controller range
Various models available: 16 ccm – 36 lpm (headspace)
Accuracy of flow rate controllers
± 5% full scale
Mass flow controllers (optional)
2 max.
Flow rates of mass flow controllers
Various models available: 3 ccm – 3 lpm (headspace)
Precision of mass flow controllers
± 1% full scale
Pump Module Built-in Pumps Fixed speed (on | off controlled)
Watson Marlow 114, Fast Load pump head
– Speed 5 rpm Flow rate (tubing wall thickness 1.6 mm)
ID: 0.5 mm: 0–0.1 ml/min ID: 0.8 mm: 0.05–2.4 ml/min ID: 1.6 mm: 0.01–0.7 ml/min ID: 2.4 mm: 0.03–1.5 ml/min ID: 3.2 mm: 0.05–2.4 ml/min ID: 4.8 mm: 0.09–4.3 ml/min
– Speed 44 rpm Flow rate (tubing wall thickness 1.6 mm)
ID: 0.5 mm: 0.02–0.9 ml/min ID: 0.8 mm: 0.04–1.8 ml/min ID: 1.6 mm: 0.12–6.2 ml/min ID: 2.4 mm: 0.26–12.8 ml/min ID: 3.2 mm: 0.41–20.7 ml/min ID: 4.8 mm: 0.75–37.4 ml/min
Speed-controlled
Watson Marlow 114, Fast Load pump head
– Speed 0.15–5 rpm Flow rate (tubing wall thickness 1.6 mm)
ID: 0.5 mm: 0–0.1 ml/min ID: 0.8 mm: 0.01–0.2 ml/min ID: 1.6 mm: 0.02–0.7 ml/min ID: 2.4 mm: 0.04–1.5 ml/min ID: 3.2 mm: 0.07–2.4 ml/min ID: 4.8 mm: 0.13–4.3 ml/min
– Speed 5–150 rpm Flow rate (tubing wall thickness 1.6 mm)
ID: 0.5 mm: 0.1–3 ml/min ID: 0.8 mm: 0.2–6 ml/min ID: 1.6 mm: 0.7–21 ml/min ID: 2.4 mm: 1.45–43.5 ml/min ID: 3.2 mm: 2.35–70.5 ml/min ID: 4.8 mm: 4.25–127.5 ml/min
External Pumps Speed-controlled
Watson Marlow 120, Fast Load pump head, up to 200 rpm
Temperature Control Module Heating and Cooling
Heating Only
For UniVessel® Glass Single-wall Culture Vessels Electrical heating system and automatic cooling water valve; connection to heating blanket and cooling finger
–
Temperature control of 8°C above cooling-water inlet temperature up to 60°C
–
Heating blanket capacities 1 L | 2 L | 5 L | 10 L: 100 | 170 | 400 | 780 W
–
For UniVessel® Glass Double-wall (Jacketed) Culture Vessels Open thermostat system with recirculation pump and automatic cooling water valve
–
Temperature control of 8°C above cooling-water inlet temperature of up to 80°C – Heating capacity: 600 W
–
For UniVessel® SU Single-use Culture Vessels Open thermostat system with recirculation pump and automatic cooling water valve; connection to heating | cooling jacket
Electrical heating blanket
Temperature control up to 50°C
Temperature control up to 50°C Heating capacity 2L : 200 W
For BIOSTAT® RM Rocking-motion Bioreactor Open thermostat system with recirculation pump and automatic cooling water valve
Electrical heating plates
Temperature control of 8°C above cooling-water inlet temperature of up to 40°C
Temperature control from room temperature up to 40°C
Heating capacity: 600 W
Heating plates: 2 + 140 W
Culture Vessel UniVessel® Glass Autoclavable Single-wall or Double-wall (Jacketed) Glass Vessel
1L
Material
Borosilicate glass, stainless steel AISI 316L, EPDM
Sizes [L]
1
2
5
10
Total volume [L]
1.6
3
6.6
13
Working volume [L]
0.4 –1
0.6 – 2
0.6 – 5
1.5 | 5 –10
Top port 19 mm | 12 mm | 6 mm
3 | 2 | 6
3 | 2 | 9
3 | 3 | 8
5 | 2 | 9
UniVessel®, Single-wall
1L
2L
5L
10 L
Weight1 [kg]
6
7
12
19
Space requirements in autoclave [W + H + D mm]
200 + 540 + 270
230 + 600 + 300
260 + 730 + 340
330 + 860 + 420
480 + 410 + 300
530 + 510 + 410
540 + 630 + 420
Space requirements in autoclave, reduced2 [W + H + D mm] ®
2
5L
10 L
UniVessel , Double-wall
1L
2L
5L
10 L
Weight1 [kg]
7
8
14
23
Space requirements in autoclave [W + H + D mm]
330 + 540 + 270
380 + 600 + 300
410 + 730 + 340
450 + 860 + 420
480 + 410 + 300
530 + 510 + 410
540 + 630 + 420
Space requirements in autoclave, reduced2 [W + H + D mm] 1
2L
With tripod and head plate, without medium Adapter for exhaust cooler to reduce height in the autoclave optionally available
Culture Vessel UniVessel® SU Single-use vessel made of pre-sterilized polycarbonate for cell culture applications Total volume [L]
2.6
Working volume [L]
0.6–2
Max. temperature
50 °C
Operating pressure
< 0.5 barg
Basic Configurations The BIOSTAT® B is a highly flexible and modular system that can be individually adapted to the requirements of your application. Below you will find an overview of the basic equipment which you can extend with diverse options. Please your Sartorius Stedim Field Representative or Application Specialist for additional information. Microbial Packages for UniVessel® Glass Volume Single Twin Digital controller, color display with touch screen Control temperature, pH, DO, stirrer speed Maintenance-free, quiet motor Storage tray for accessories Aeration module with 2 solenoid valves 2 flow rate controllers for manual flow rate control (AIR | N2, O2) Software configured for microbial applications 4-stage DO cascade BioPAT® MFCS software for data acquisition 2 integrated pumps for pH control (acid | base) Temperature control module for double-wall vessels 2 external signals 0 –10 V Standard test and documentation Installation set for the gas and water connections Power cable Double-wall culture vessel Stirrer shaft with single-mechanical seal and direct coupling 2 addition bottles for correction agents Exhaust cooler Aeration filters Rushton impellers Ring sparger 4-way addition port Inoculation port Harvest pipe, height-adjustable Manual sampler Tool set for disassembly of vessel Pt100 temperature sensor pH sensor DO sensor, amperometric
Cell Culture Packages for UniVessel® Glass Volume Single Twin Digital controller, color display with touch screen Control temperature, pH, DO, stirrer speed Maintenance-free, quiet motor Storage tray for accessories Aeration module with 4 solenoid valves 5 flow rate controllers for manual flow rate control (AIR Overlay, AIR sparger, N2, O2, CO2) Software configured for cell culture applications 4-stage DO cascade BioPAT® MFCS software for data acquisition 1 integrated pump for pH control (base) Temperature control module for single-wall vessels 2 external signals 0 –10 V Standard test and documentation Installation set for the gas and water connections Power cable Single-wall culture vessel with tripod Heating blanket 120 | 230 V Stirrer shaft with single mechanical seal and direct coupling 1 addition bottle for correction agent Exhaust cooler Aeration filters 3-blade segment impeller Microsparger 4-way addition port Harvest pipe, height-adjustable Manual sampler Tool set for vessel dismantling Pt100 temperature sensor pH sensor DO sensor, amperometric
Options Control System Advanced DO controller Flexible switching CO2 from sparger to headspace Software for dual use MO | CC Mass flow controller Antifoam control via sensor Mechanical foam destroyer (MO applications) Level control via sensor Weight measurement substrates | culture vessel Gravimetric feed | level control Substrate addition via time profile Redox (ORP) measurement Turbidity measurement BioPAT® Xgas O2 | CO2 off-gas analysis System IQ | OQ BioPAT® MFCS SCADA system UniVessel® Glass Double-wall culture vessel Magnetic coupling for drive motor STT connectors for safe inoculation and media transfer Cooling finger (single-wall culture vessels) Spin filter for perfusion mode Aeration basket for organisms sensitive to shear stress Rushton impellers | 3-blade segment impellers Baffles Bottle holder Adapter for height reduction of exhaust cooler for the autoclave Inoculation port 3-way addition port Universal adapter ID 3.2 mm Harvest pipe bent for complete draining Port adapter 19 mm – 12 mm Set of consumables
Sales Salesand andService Services s Sales les Sales and Sales and and Service Service Service and Service s s s s For further s, visit www.sartorius-stedim.com For further s, visit www.sartorius-stedim.com
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B Avenue de Jouques – –CSCS91051 Sartorius do Malaysia Avenue de Jouques 91051 Sartorius doBrasil BrasilLtda Ltda Malaysia Malaysia 199155, Saint-Petersburg 199155, Saint-Petersburg Sartorius nue venue Avenue de Jouques deAvenue deJouques Jouques –de CS –Jouques –CS 91051 CS91051 91051 – CS 91051 199155, Sartorius Sartorius do do Sartorius Brasil doBrasil Brasil Ltda Ltda do Ltda Brasil Ltda Malaysia Malaysia Malaysia 199155, 199155, Saint-Petersburg Saint-Petersburg 199155, Saint-Petersburg Saint-Petersburg 13781 Aubagne Cedex Avenida Senador Vergueiro 2962 Sartorius Stedim Malaysia Sdn. 13781 Aubagne Cedex Avenida Senador Vergueiro 2962 Sartorius Stedim Malaysia Sdn 81 3781 13781 Aubagne Aubagne Aubagne 13781 Cedex Cedex Aubagne Cedex Cedex Avenida Avenida Avenida Senador Senador Avenida Senador Vergueiro Vergueiro Senador Vergueiro 2962 Vergueiro 2962 2962 2962 Sartorius Sartorius Sartorius Stedim Stedim Sartorius Stedim Malaysia Malaysia Malaysia Stedim Sdn. Sdn. Malaysia Sdn. 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Phone +7.812.327.5.327 Phone +7.812.327.5.327 Bernardo dodoCampo Lot São Bernardo Campo LotLot LotL3-E-3B, L3-E-3B, Enterprise Phone Phone Phone +7.812.327.5.327 +7.812.327.5.327 +7.812.327.5.327 Phone +7.812.327.5.327 SãoSão Bernardo SãoBernardo Bernardo São doSão Bernardo do Campo do Campo Campo do Campo L3-E-3B, LotL3-E-3B, L3-E-3B, Lot Enterprise Enterprise L3-E-3B, Enterprise 4 Enterprise 4 4 Enterprise 4 44 Phone +33.442.845600 Phone+33.442.845600 +33.442.845600 ne hone Phone +33.442.845600 +33.442.845600 +33.442.845600 Phone Fax +7.812.327.5.323 Fax +7.812.327.5.323 - -SPTechnology 09600-000 SP-Brasil BrasilTechnology Technology ParkMalaysia Malaysia FaxFax +7.812.327.5.323 Fax+7.812.327.5.323 +7.812.327.5.323 Fax +7.812.327.5.323 CEPCEP 09600-000 CEP09600-000 09600-000 CEPCEP -CEP 09600-000 SP-09600-000 -SPBrasil SP-Brasil Brasil - SPBrasil Technology Technology Park Technology Park Park Malaysia Malaysia Malaysia Park Park Malaysia Fax +33.442.845619 Fax+33.442.845619 +33.442.845619 +33.442.845619 ax Fax+33.442.845619 +33.442.845619 Fax Bukit Jalil Bukit Bukit JalilJalil Jalil BukitBukit Jalil Jalil Phone +55.11.4362.8900 Phone +55.11.4362.8900 Bukit Phone Phone Phone +55.11.4362.8900 +55.11.4362.8900 +55.11.4362.8900 Phone +55.11.4362.8900 57000 Kuala Lumpur, Malaysia 57000 Kuala Lumpur, Malaysia 57000 57000 57000 Kuala Kuala Kuala 57000 Lumpur, Lumpur, Lumpur, Kuala Malaysia Malaysia Lumpur, Malaysia Malaysia Spain Spain ++55.11.4362.8901 55.11.4362.8901 Spain Spain Spain FaxFax +Fax 55.11.4362.8901 + +55.11.4362.8901 55.11.4362.8901 FaxFax +Fax 55.11.4362.8901 Sartorius Stedim SAS Sartorius Stedim SAS Spain orius artorius Sartorius Stedim Stedim Sartorius Stedim Stedim SASSAS SAS SAS Sartorius Stedim Spain, S.A.U. 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Avda. de la dede Industria, laAvda. la Industria, Industria, dede 32 la 32 32 323232 FaxFax +60.3.8996.0755 Fax+60.3.8996.0755 +60.3.8996.0755 Fax +60.3.8996.0755 de Jouques 71058 Mexico Avenue de Jouques –CSCS 71058 Mexico nue venue Avenue de Jouques deAvenue Avenue deJouques Jouques –de CS –Jouques –CS 71058 CS71058 71058 ––CS 71058 Mexico Mexico Mexico Mexico Edificio PAYMA Edificio PAYMA Edificio Edificio Edificio PAYMA PAYMA Edificio PAYMA PAYMA 13781 Aubagne Cedex Sartorius dede México S.A. dedeC.V. 13781 Aubagne Cedex Sartorius México S.A. C.V. 81 3781 13781 Aubagne Aubagne Aubagne 13781 Cedex Cedex Aubagne Cedex Cedex Sartorius Sartorius Sartorius de México de Sartorius de México México S.A. de S.A. de S.A. México C.V. de de C.V. C.V. 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Longmead Longmead Longmead Business Longmead Business Business Centre Centre Business Centre Centre Longmead Business Centre Longmead Business Centre Unit 0800 1800 Vilvoorde Vilvoorde Vilvoorde 1800 Vilvoorde Unit Unit 5, 7-11 5, 5, 7-11 7-11 Unit Rodeo Rodeo Rodeo 5, Drive 7-11 Drive Drive Rodeo Drive 1800 1800Vilvoorde Vilvoorde Unit Unit5,5,7-11 7-11Rodeo RodeoDrive Drive Phone Blenheim Blenheim Blenheim Road, Blenheim Road, Road, Epsom Epsom Epsom Road, Epsom Phone +82.31.622.5700 Phone Phone +82.31.622.5700 +82.31.622.5700 +82.31.622.5700 Blenheim Road, Epsom Blenheim Road, Epsom Phone +82.31.622.5700 Phone +82.31.622.5700 Dandenong Dandenong Dandenong South Dandenong South South Vic Vic 3175 Vic South 3175 3175 Vic 3175 Dandenong DandenongSouth SouthVic Vic3175 3175FaxFax Surrey Surrey Surrey KT19 KT19 KT19 9Surrey QQ 9 9QQ QQ KT19 9 9QQ Fax +82.31.622.5799 ne hone Phone +32.2.756.06.80 +32.2.756.06.80 +32.2.756.06.80 Phone +82.31.622.5799 Fax+82.31.622.5799 +82.31.622.5799 Surrey KT19 Surrey KT19 9QQ QQ Phone +32.2.756.06.80 Phone+32.2.756.06.80 +32.2.756.06.80 Fax Fax+82.31.622.5799 +82.31.622.5799 +32.2.756.06.81 ax Fax+32.2.756.06.81 +32.2.756.06.81 Fax Phone Phone Phone +61.3.8762.1800 +61.3.8762.1800 +61.3.8762.1800 Phone +61.3.8762.1800 Fax +32.2.756.06.81 Fax+32.2.756.06.81 +32.2.756.06.81 Phone Phone Phone +44.1372.737159 +44.1372.737159 +44.1372.737159 Phone +44.1372.737159 Phone +61.3.8762.1800 Phone +61.3.8762.1800 Phone +44.1372.737159 Phone +44.1372.737159 FaxFax +61.3.8762.1828 Fax +61.3.8762.1828 +61.3.8762.1828 Fax +61.3.8762.1828 FaxFax +44.1372.726171 Fax+44.1372.726171 +44.1372.726171 Fax +44.1372.726171 Fax Fax+61.3.8762.1828 +61.3.8762.1828 Fax +44.1372.726171 Fax +44.1372.726171 gary Hungary ungary Hungary Hungary Hungary orius artorius Sartorius Stedim Stedim Sartorius Stedim Hungária Hungária Hungária Stedim Kft.Hungária Kft. 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LLC “Biohit” LLC “Biohit” 2092 2092 Budakeszi Budakeszi Budakeszi 2092 Budakeszi No.No. 33 No.33 Yu’an 33Yu’an Yu’an No. Road Road 33 Road Yu’an Road Post Post Post Box Box 440 Box 440 Post “B” 440 “B” Box “B” 440 “B” 2092 2092Budakeszi Budakeszi No. 33 Yu’an Road No. 33 Yu’an Road Post Box 440 “B” Post Box 440 “B” Airport Airport Airport Industrial Industrial Industrial Airport Park Industrial Park Park Zone Zone Zone B Park B B Zone B 01001 01001 01001 Kiev, Kiev, Kiev, Ukraine 01001 Ukraine Ukraine Kiev, Ukraine ne hone Phone +36.23.457.227 +36.23.457.227 +36.23.457.227 Phone +36.23.457.227 Airport Industrial Park Zone Airport Industrial Park ZoneBB 01001 01001Kiev, Kiev,Ukraine Ukraine Shunyi Shunyi Shunyi District, District, District, Shunyi Beijing Beijing District, Beijing 101300 101300 101300 Beijing 101300 Phone +36.23.457.227 Phone +36.23.457.227 +36.23.457.147 ax Fax+36.23.457.147 +36.23.457.147 Fax +36.23.457.147 Shunyi ShunyiDistrict, District,Beijing Beijing101300 101300 Phone Phone Phone +380.44.411.4918 +380.44.411.4918 +380.44.411.4918 Phone +380.44.411.4918 Fax Fax+36.23.457.147 +36.23.457.147 Phone +380.44.411.4918 Phone +380.44.411.4918 Phone Phone Phone +86.10.80426516 +86.10.80426516 +86.10.80426516 Phone +86.10.80426516 FaxFax +380.50.623.3162 Fax+380.50.623.3162 +380.50.623.3162 Fax +380.50.623.3162 Phone Phone+86.10.80426516 +86.10.80426516 Fax Fax +86.10.80426580 Fax +86.10.80426580 +86.10.80426580 Fax +86.10.80426580 Fax +380.50.623.3162 Fax +380.50.623.3162 Italy aly Italy Fax Fax+86.10.80426580 +86.10.80426580 Italy Italy orius artorius Sartorius Stedim Stedim Sartorius Stedim Italy Italy Italy S.p.A. Stedim S.p.A. S.p.A. Italy S.p.A. Sartorius Stedim Italy Sartorius Stedim ItalyS.p.A. S.p.A. dell’Antella, ia Viadell’Antella, dell’Antella, Via 76/A dell’Antella, 76/A 76/A 76/A Sartorius Sartorius Sartorius Stedim Stedim Sartorius Stedim Biotech Biotech Biotech Stedim (Beijing) (Beijing) (Beijing) Biotech Co.Co. Ltd. (Beijing) Co.Ltd. Ltd. Co. Ltd. Via 76/A Viadell’Antella, dell’Antella, 76/A Sartorius Stedim Biotech Sartorius Stedim Biotech(Beijing) (Beijing)Co. Co.Ltd. Ltd. 12 0012 50012 Antella-Bagno Antella-Bagno Antella-Bagno 50012 Antella-Bagno a Ripoli a aRipoli Ripoli (FI) (FI) a(FI) Ripoli (FI) Shanghai Shanghai Shanghai Branch Shanghai Branch Branch Office Office Office Branch Office 50012 50012Antella-Bagno Antella-Bagnoa aRipoli Ripoli(FI) (FI) Shanghai Branch Shanghai Branch 3rd3rd Floor, 3rdFloor, Floor, North 3rd North North Wing, Floor, Wing, Wing, North Tower Tower Tower Wing, 1 1Office 1Office Tower 1 ne hone Phone +39.055.63.40.41 +39.055.63.40.41 +39.055.63.40.41 Phone +39.055.63.40.41 3rd Floor, North Wing, 3rd Floor, North Wing,Tower Tower1 1 No.No. 4560 No.4560 4560 Jin Jin No. Ke Jin Ke Road 4560 KeRoad Road Jin Ke Road Phone +39.055.63.40.41 Phone +39.055.63.40.41 +39.055.63.40.526 ax Fax+39.055.63.40.526 +39.055.63.40.526 Fax +39.055.63.40.526 No. Jin Road No.4560 4560 JinKe Ke Road 201210 Pudong Pudong Pudong District, District, Pudong District, Shanghai Shanghai District, Shanghai 201210 Shanghai 201210 201210 Fax Fax+39.055.63.40.526 +39.055.63.40.526 Pudong PudongDistrict, District,Shanghai Shanghai201210 201210 Phone Phone Phone +86.21.68782300 +86.21.68782300 +86.21.68782300 Phone +86.21.68782300 Phone +86.21.68782300 +86.21.68782332 |+86.21.68782300 68782882 FaxFax Fax+86.21.68782332 +86.21.68782332 FaxPhone +86.21.68782332 | 68782882 | 68782882 | 68782882 +86.21.68782332| 68782882 | 68782882 Fax Fax+86.21.68782332 Sartorius Sartorius Sartorius Stedim Stedim Sartorius Stedim Biotech Biotech Biotech Stedim (Beijing) (Beijing) (Beijing) Biotech Co.Co. Ltd. (Beijing) Co.Ltd. Ltd. Co. Ltd. Guangzhou Guangzhou Guangzhou Representative Guangzhou Representative Representative Office Office Office Office Sartorius Stedim Biotech (Beijing) SartoriusRepresentative Stedim Biotech (Beijing)Co. Co.Ltd. Ltd. 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Specifications subject to change without notice. Printed in the EU on paper bleached without chlorine. | W Publication No.: SBI1513-e141106 · Order No.: 85032-542-70 · Ver. 11 | 2014
Asia|Pacific Asia|Pacific Asia|Pacific Asia|Pacific Asia Asia||Pacific Pacific
BIOSTAT® B at a Glance Our BIOSTAT® B is the ideal benchtop bioreactor for your lab. The multi-talented control tower opens up a new world of flexibility for your changing requirements. Use it as single or twin configuration, chose your cultivation chamber from our proven range of options: – Conventional stirred tank glass vessels – Single-use stirred tank vessel Univessel® SU – Wave-mixed Flexsafe RM bags
Applications – Process development and optimization – Scale-up and scaledown studies – Seed expansion – Cell bank production – Protein supply
Cells – Mammalian – Insect – Microbial – Yeast – Fungi
Industries – Biopharmaceuticals – Vaccines – Cell therapies – Industrial biotechnology – Basic research – Education
Process Modes – Batch – Fed-batch – Continuous – Perfusion
Proven Technology With several thousand installations in over 50 countries, BIOSTAT® B is the most successful benchtop bioreactor in the world. Benefit from our experience and collaboration with customers worldwide.
Configurable Design your Sartorius representative and configure your BIOSTAT® B bioreactor solution matching your specific needs. Benefit from our flexible and scalable options.
Reliable Quality Every BIOSTAT® B is thoroughly tested before it leaves our production facilities in . Benefit from our global service and application specialist network for professional installation and training.
UniVessel® Glass Our proven autoclavable borosilicate glass culture vessel is available in four different volumes: 1 L, 2 L, 5 L and 10 L, for all kinds of cell culture and microbial applications.
Benefits −− Classic stirred-tank design simplifies your scale-up and scale-down studies −− Extensive performance and engineering data package available −− Save additional investment and use our special design for small autoclaves
Fully interchangable glass and single-use stirred-tank culture vessels.
UniVessel® SU Our single-use 2 L stirred tank vessel for cell culture applications combines scalable design with all benefits of disposable solutions. No more worries about challenging timelines, workload or cross-contamination. Get started within minutes.
Benefits −− Fully single-use vessel, DO and pH sensors −− Proven, scalable stirred tank design −− Connection kit to upgrade existing bioreactor controllers
BIOSTAT® B – The Universal Controller for Your Lab
Main Applications −− Mammalian, insect, microbial and plant cell culture −− Process development, optimization, characterization −− Seed and small scale production −− Protein supply
Main Applications −− Mammalian, insect and plant cell culture −− Process development, optimization, characterization −− Seed and small scale production −− Production of cell banks
Watch Video: www.sartorius.com/ video-biostat-b
Main Applications −− Mammalian, insect, plant and low density microbial culture −− Seed and small scale production −− Protein supply −− Stem cell expansion and production
RM Rocker Our proven wave-mixed bioreactor consists of a rocker unit with bag holder and pillow-shaped Flexsafe RM single-use bags for culture volumes from 100 mL to 25 L. Use it as basic system or in combination with the BIOSTAT® B control tower and single-use optical pH and DO sensors to enable advanced control strategies for batch, fed-batch and continuous perfusion.
Benefits −− Optimized film formula, traceability of raw materials and control of the entire Flexsafe RM bag manufacturing process assure excellent and consistent cell growth of the most sensitive cell lines −− Space-saving, individual control of two RM bags on the same rocker platform −− Easy-to-use rocker with advanced alarms and safety features for reliable cultivation
12” Touch Screen with closed frame; protected against water splashes and dust deposits. Easy-to-use and reliable operation of your BIOSTAT® B due to intuitive design of human-machine interface and advanced touchscreen technology – even while wearing laboratory gloves.
New Standards for Interference-Free Measurements All inlets and ports for, e.g. cooling water, process gasses, electricity, ethernet and potential-free alarm are located on the rear of the control tower. An equipotential bonding conductor shields the bioreactor against electromagnetic current and ensures interference-free measurements during your processes.
Control Tower The control tower contains both the aeration, pump and temperature control modules, saving valuable bench space in your lab.
BIOSTAT® B Configurable Flexibility Single | Twin Control Tower One BIOSTAT® B control tower can control up to two vessels completely independently to save valuable lab space. Select culture vessel types and volumes from our range of options, e.g. glass vessel, single-use vessel or wave-mixed Flexsafe® RM bags.
Fast Load Peristaltic Pumps Insert the tubing in seconds: Open, insert tube, close, done! No more pinched fingers or torn gloves. Up to four internal pumps can be selected per vessel. Choose between speedcontrolled pumps and fixedspeed pumps for feed and corrective agent addition.
Connections for Sensors and External Accessories Quick-connect couplings make it easy to attach all cables and supplies to the culture vessels. All connections are clearly marked with the same names used on the rear control tower and in the local control software menu for fast, error proof identification.
Storage Tray A storage tray for accessories helps you organize your workplace.
Aeration Strategies A series of flexible aeration options turns the BIOSTAT® B into a multitalented bioreactor for a wide range of different applications, including high-cell density microbial fermentation requiring considerable amounts of oxygen to high-cell density cell culture applications requiring removal of excessive carbon dioxide.
Microbial Applications in Combination with UniVessel® Glass Various controller and hardware configurations enable aeration strategies using air or oxygen or classical O2 enrichment of air. For anaerobic processes, the air inlet can be used for nitrogen. The standard built-in solenoid valves in combination with a flow meter ensure reliable gas supply for simple applications. Optional mass flow controllers provide exact flow rate control of individual gases. In combination with BioPAT® Xgas off-gas analysis, this allows mass balancing consumed and produced gases.
Gas Inlet
Solenoid Valve MFC* (max. 2) (max. 2)
Air
Flow Meter
Gas Outlet
Sparger
O2 *MFC = mass flow controller
Cell Culture and Multi-Purpose Applications in Combination with UniVessel® Glass and UniVessel® SU Gas Inlet
Solenoid Valve MFC * (4 max.) (4 max.)
Flow Meter
Gas Outlet
Overlay
Air
Sparger
O2 N2 CO2 Switching CO2 from sparger to overlay *MFC = mass flow controller
Five different gas lines, equipped with solenoid valves and|or up to four optional mass flow controllers and flow meters deliver ultimate flexibility and accuracy. The BIOSTAT® B allows you to optionally switch between sparger and overlay supply of CO2 for pH control. In addition, a constant air | CO2 mixture can be created in the overlay line to reproduce conditions like those prevailing in a CO2 incubator.
Cell Culture Applications in Combination with Our Rocking-Motion Bioreactor The wave generated in the RM bags due to the rocking motion ensures effective gas exchange through the gas-liquid interface. Four different gas lines for air, O2, N2 and CO2 are equipped with flow meters and four high-precision mass flow controllers. An integrated pressure sensor continuously measures the pressure inside the bag. To ensure process safety, aeration is switched off when an upper threshold is reached.
Gas Inlet
MFC*
Flow Meter
Gas Outlet
Overlay
Air O2 N2 CO2 *MFC = mass flow controller
You can configure your BIOSTAT® B aeration system in a similar way as your BIOSTAT® STR single-use stirred tank bioreactor. Get ready for seamless scale-up and -down, from laboratory scale to large scale single-use production.
DCU – Local Control Since we introduced the first DCU-controlled bioreactors back in the late 80’s, we have installed thousands of such bioreactors to date at leading companies in the pharmaceutical and biotech industry worldwide. We have continuously improved our robust, intuitive-use and industry-proven DCU control technology, now available in its fourth generation. It is our standard local control platform for our BIOSTAT® bioreactors, SARTOFLOW® crossflow filtration units and FlexAct® configurable systems available for a large number of unit operations. The intuitive touch screen on the control tower is used to locally operate the BIOSTAT® B: Aeration control
Balances and gravimetric flow control
Overview of process parameters
Pump module
Submenus
Overview and close-up Culture vessels
Status display
Automatic Feed Control and Continuous Processing Design your process strategy or select different options. Configure your BIOSTAT® B with gravimetric feed control, gravimetric level control or substrate addition profiles. This enables you to run your BIOSTAT® B in batch, fed-batch, continuous or perfusion mode.
Automatic DO Control Besides classic DO cascade control, we have developed the unique advanced DO controller that gives you more flexibility to develop and optimize your DO control strategy. The advanced DO controller s parallel adjustment of all DO affecting parameter settings like stirrer speed and gas flow rates of air and pure oxygen, automatically and simultaneously to control the DO set point. Optionally, you can keep the total gas flow rate constant and the ratio of pure oxygen in the gas mixture is increased automatically to match the oxygen consumption of the culture. This gives you ultimate flexibility to adapt your aeration strategy to your process requirements.
Automatic pH Control Control the pH of your culture by automatic acid and base addition or by CO2 aeration and base addition. If you want to use your BIOSTAT® B for both microbial and cell culture you can configure your bioreactor system with a combined acid control via CO2 aeration and acid addition.
CO2
Base Acid
BioPAT® MFCS – SCADA Process Control BioPAT® MFCS is a multi-functional supervisory control and data acquisition software, specifically developed for bioprocessing applications in upstream and downstream. Every BIOSTAT® bioreactor comes with basic data recording functionality of our BioPAT® MFCS. Rely on more than 7,000 installations worldwide and over 25 years of experience. – Multi- network access for up to 16 process units – On-line and off-line sample data management, visualization, evaluation – Remote monitoring, control and alarming
BioPAT® MFCS | win In addition to providing a genuine SCADA system for conventional and single-use bioprocess applications, the optional advanced version of BioPAT® MFCS includes a series of add-on modules: – Bioprocess optimization with Design of Experiments module (DoE) – Automation with recipes according to ANSI | ISA-88.01 – 21 CFR, Part 11 compatibility – Secure integration into company networks
– Flexible device connection facility through OPC – Expanded data recording and data archiving capabilities – Alarm recording and transmission to multimedia devices
Our Mission Is Your Productivity We are well aware of how important smooth-running equipment is for effective process development and efficient production. That is why we at Sartorius offer you a broad range of preventive technical service, repair and application specialist . Our ISO certified quality management system ensures that you receive equipment and services that meet the strictest quality requirements. Become one of our many customers who benefit from our local presence worldwide, highly qualified specialists and well-versed technicians.
Services Offered for BIOSTAT® B −− On-site installation and application specialist , ensuring that your equipment is perfectly up and unning right from the start −− Factory acceptance and site-acceptance testing −− On-site preventive maintenance and repair −− Consumable and spare part kits
Technical Specifications BIOSTAT® B Control Tower Weight Single | Twin
~ 40 | 55 kg (88 | 121 lbs.)
Control Tower Dimensions (W + H + D) Single | Twin
410 + 810 + 520 mm (16“ + 32“ + 20“)
Utility Connections Power supply
– 230 V (± 10%), 50 Hz, max. power consumption 10 A – 120 V (± 10%), 60 Hz, max. power consumption 12 A – Potential equalisation
International protection rating
IP21
Gases
– Gas supply pressure, 1.5 barg – Dry, oil and dust-free – Hose barb for tubing, external dia. = 6 mm
Water
– Water supply pressure, 2 – 8 barg – Flow rate up to 20 lpm – Temperature min. = 4°C – Discharge pressure-less – Hose barb for tubing, external dia. = 10 mm – Degree of hardness: 12 dH max.
Control Tower Housing
Stainless steel, AISI 304
Display
Touch screen, 12”, glass, capacitive
Resolution
125 dpi
SCADA communication
Industrial Ethernet
Potential-free (common) alarm
•
Safety valve gas pressure
1 bar (14.5 psi) for UniVessel® Glass and SU | 100 mbar for RM
Water inlet pressure reduction
1.5 bar (22 psi), integrated pressure control
®
Motor Drive (Univessel Glass | SU) Maintenance-free, quiet direct drive
Power: 200 W
Maintenance-free top drive with magnetic motor coupling
Power: 200 W
Rotation speed motor, direct coupling
1L: 20 – 2,000 rpm 2L: 20 – 2,000 rpm 5L: 20 – 1,500 rpm 10L: 20 – 800 rpm 2L SU: 20 – 400 rpm
Rocker Platform Power supply
– 230 V (± 10%), 50 Hz, power consumption 10 A – 120 V (± 10%), 60 Hz, power consumption 12 A
Power consumption
600 W
International protection rating
IP23
Rocking speed
8 – 42 ± 1 (r/min)
Rocking angle
4 –10 ± 0.3 (°)
Process Control | Sensors UniVessel® Glass
UniVessel® SU
Rocking Motion
Pt100 | 0–150°C (temperature control 0-80°C) | 0.1°C
•
•
•
Dissolved oxygen, reusable
Polarographic or optical | 0–100 % | 0.1 %
•
•
Dissolved oxygen, single-use
Range: 0 –100 % Precision: 0.1%
pH, reusable
Combined measuring electrode Range: 2–12 pH Precision: 0.01 pH
pH, single-use
Range: 6.5 - 8.5 pH Precision: 0.1 pH
Foam control
Electrical conductive sensor, stainless steel, ceramic insulated
•
Level
Electrical conductive sensor, stainless steel, ceramic insulated
•
Turbidity
1-channel NIR absorption sensor | 0–6 AU | 0,01 AU
•
Redox
Combined measurement with pH sensor | -1,000 – 1,000 mV | 1 mV
•
•
Balance substrate
7 kg max. | 1 g 60 kg max. | 2 g 300 kg max. | 100 g
•
•
Balance culture vessel
60 kg max. | 2 g
•
•
RM load cells
30 kg max. | 10 g Weight: 9 kg Dimensions: 609 | 536 | 60-68 mm 100–240 V VAC | 24 VDC | 15 W
External signal input
0–10 V or 4-20 mA UniVessel® Glass | SU: 4 max. Rocker: 2 max.
Sensor | Measurement Range | Display Accuracy Temperature
• •
•
• •
•
•
•
•
•
•
Aeration Module Outlet to culture vessel | bag ®
UniVessel Glass MO (Microbial)
Hose coupling d external = 6 mm Two-gas mixing with sparger outlet
AIR with O2 Enrichment or Gas Flow Ratio mixing along with anaerobic fermentation; for further information, please see page on “aeration strategies” Max. total flow
Up to 20 l/min total volume flow
Flow rate controllers
2
Flow rate controller range
Various models available: 0.1-20 liters per min. [lpm] (sparger)
Accuracy of flow rate controllers
± 5 % full scale
Mass flow controllers (optional)
2 max.
Flow rates of mass flow controllers
Various models available: 0.03-20 lpm (sparger)
Accuracy of mass flow controllers
± 1 % full scale
®
UniVessel Glass CC (Cell Culture) | UniVessel® SU
Four-gas mxing with sparger and headspace outlet
Additive flow, 4-gas (Air, O2, N2, CO2) mixture; for further information, please see page on “Aeration Strategies” Max. total flow
Up to 13 l/min total volume flow
Number of flow rate controllers
5
Flow rate controller range
Various models available: 3.3 ccm – 1.6 lpm (sparger) 0.16 lpm – 13 lpm (headspace)
Accuracy of flow rate controllers
± 5 % FS
Mass flow controllers (optional)
4 max.
Flow rates of mass flow controllers
Various models available: 1 ccm – 1.5 lpm (sparger) 0.03 lpm – 10 lpm (headspace)
Precision of mass flow controllers
± 1 % FS
UniVessel® Glass, Dual Use
Four-gas mixing with sparger and headspace outlet
Additive flow, 4-gas (Air, O2, N2, CO2) mixture; for further information, please see page on “Aeration Strategies” Max. total flow
Up to 20 l/min total volume flow
Flow rate controllers
5
Flow rate controller range
Various models available: 3.3 ccm – 20 lpm (sparger) 50 ccm – 20 lpm (headspace)
Accuracy of flow rate controllers
± 5% full scale
Mass flow controllers (optional)
4 max.
Flow rates of mass flow controllers
Various models available: 0.6 ccm – 20 lpm (sparger) 10 ccm – 20 lpm (headspace)
Precision of mass flow controllers
± 1 % FS
Rocker
Four-gas mixing with sparger and headspace outlet
Additive flow, 4-gas (Air, O2, N2, CO2) mixture; for further information, please see page on “Aeration Strategies” Max. total flow
Up to 10 l/min total volume flow
Max. CO2 flow
One bag: 1 + 1 lpm Twin bag: 2 + 0.5 lpm
CO2 concentration controller
0.8 % – 15% | ± 5%
Flow rate controllers
4
Flow rate controller range
Various models available: 16 ccm – 36 lpm (headspace)
Accuracy of flow rate controllers
± 5% full scale
Mass flow controllers (optional)
2 max.
Flow rates of mass flow controllers
Various models available: 3 ccm – 3 lpm (headspace)
Precision of mass flow controllers
± 1% full scale
Pump Module Built-in Pumps Fixed speed (on | off controlled)
Watson Marlow 114, Fast Load pump head
– Speed 5 rpm Flow rate (tubing wall thickness 1.6 mm)
ID: 0.5 mm: 0–0.1 ml/min ID: 0.8 mm: 0.05–2.4 ml/min ID: 1.6 mm: 0.01–0.7 ml/min ID: 2.4 mm: 0.03–1.5 ml/min ID: 3.2 mm: 0.05–2.4 ml/min ID: 4.8 mm: 0.09–4.3 ml/min
– Speed 44 rpm Flow rate (tubing wall thickness 1.6 mm)
ID: 0.5 mm: 0.02–0.9 ml/min ID: 0.8 mm: 0.04–1.8 ml/min ID: 1.6 mm: 0.12–6.2 ml/min ID: 2.4 mm: 0.26–12.8 ml/min ID: 3.2 mm: 0.41–20.7 ml/min ID: 4.8 mm: 0.75–37.4 ml/min
Speed-controlled
Watson Marlow 114, Fast Load pump head
– Speed 0.15–5 rpm Flow rate (tubing wall thickness 1.6 mm)
ID: 0.5 mm: 0–0.1 ml/min ID: 0.8 mm: 0.01–0.2 ml/min ID: 1.6 mm: 0.02–0.7 ml/min ID: 2.4 mm: 0.04–1.5 ml/min ID: 3.2 mm: 0.07–2.4 ml/min ID: 4.8 mm: 0.13–4.3 ml/min
– Speed 5–150 rpm Flow rate (tubing wall thickness 1.6 mm)
ID: 0.5 mm: 0.1–3 ml/min ID: 0.8 mm: 0.2–6 ml/min ID: 1.6 mm: 0.7–21 ml/min ID: 2.4 mm: 1.45–43.5 ml/min ID: 3.2 mm: 2.35–70.5 ml/min ID: 4.8 mm: 4.25–127.5 ml/min
External Pumps Speed-controlled
Watson Marlow 120, Fast Load pump head, up to 200 rpm
Temperature Control Module Heating and Cooling
Heating Only
For UniVessel® Glass Single-wall Culture Vessels Electrical heating system and automatic cooling water valve; connection to heating blanket and cooling finger
–
Temperature control of 8°C above cooling-water inlet temperature up to 60°C
–
Heating blanket capacities 1 L | 2 L | 5 L | 10 L: 100 | 170 | 400 | 780 W
–
For UniVessel® Glass Double-wall (Jacketed) Culture Vessels Open thermostat system with recirculation pump and automatic cooling water valve
–
Temperature control of 8°C above cooling-water inlet temperature of up to 80°C – Heating capacity: 600 W
–
For UniVessel® SU Single-use Culture Vessels Open thermostat system with recirculation pump and automatic cooling water valve; connection to heating | cooling jacket
Electrical heating blanket
Temperature control up to 50°C
Temperature control up to 50°C Heating capacity 2L : 200 W
For BIOSTAT® RM Rocking-motion Bioreactor Open thermostat system with recirculation pump and automatic cooling water valve
Electrical heating plates
Temperature control of 8°C above cooling-water inlet temperature of up to 40°C
Temperature control from room temperature up to 40°C
Heating capacity: 600 W
Heating plates: 2 + 140 W
Culture Vessel UniVessel® Glass Autoclavable Single-wall or Double-wall (Jacketed) Glass Vessel
1L
Material
Borosilicate glass, stainless steel AISI 316L, EPDM
Sizes [L]
1
2
5
10
Total volume [L]
1.6
3
6.6
13
Working volume [L]
0.4 –1
0.6 – 2
0.6 – 5
1.5 | 5 –10
Top port 19 mm | 12 mm | 6 mm
3 | 2 | 6
3 | 2 | 9
3 | 3 | 8
5 | 2 | 9
UniVessel®, Single-wall
1L
2L
5L
10 L
Weight1 [kg]
6
7
12
19
Space requirements in autoclave [W + H + D mm]
200 + 540 + 270
230 + 600 + 300
260 + 730 + 340
330 + 860 + 420
480 + 410 + 300
530 + 510 + 410
540 + 630 + 420
Space requirements in autoclave, reduced2 [W + H + D mm] ®
2
5L
10 L
UniVessel , Double-wall
1L
2L
5L
10 L
Weight1 [kg]
7
8
14
23
Space requirements in autoclave [W + H + D mm]
330 + 540 + 270
380 + 600 + 300
410 + 730 + 340
450 + 860 + 420
480 + 410 + 300
530 + 510 + 410
540 + 630 + 420
Space requirements in autoclave, reduced2 [W + H + D mm] 1
2L
With tripod and head plate, without medium Adapter for exhaust cooler to reduce height in the autoclave optionally available
Culture Vessel UniVessel® SU Single-use vessel made of pre-sterilized polycarbonate for cell culture applications Total volume [L]
2.6
Working volume [L]
0.6–2
Max. temperature
50 °C
Operating pressure
< 0.5 barg
Basic Configurations The BIOSTAT® B is a highly flexible and modular system that can be individually adapted to the requirements of your application. Below you will find an overview of the basic equipment which you can extend with diverse options. Please your Sartorius Stedim Field Representative or Application Specialist for additional information. Microbial Packages for UniVessel® Glass Volume Single Twin Digital controller, color display with touch screen Control temperature, pH, DO, stirrer speed Maintenance-free, quiet motor Storage tray for accessories Aeration module with 2 solenoid valves 2 flow rate controllers for manual flow rate control (AIR | N2, O2) Software configured for microbial applications 4-stage DO cascade BioPAT® MFCS software for data acquisition 2 integrated pumps for pH control (acid | base) Temperature control module for double-wall vessels 2 external signals 0 –10 V Standard test and documentation Installation set for the gas and water connections Power cable Double-wall culture vessel Stirrer shaft with single-mechanical seal and direct coupling 2 addition bottles for correction agents Exhaust cooler Aeration filters Rushton impellers Ring sparger 4-way addition port Inoculation port Harvest pipe, height-adjustable Manual sampler Tool set for disassembly of vessel Pt100 temperature sensor pH sensor DO sensor, amperometric
Cell Culture Packages for UniVessel® Glass Volume Single Twin Digital controller, color display with touch screen Control temperature, pH, DO, stirrer speed Maintenance-free, quiet motor Storage tray for accessories Aeration module with 4 solenoid valves 5 flow rate controllers for manual flow rate control (AIR Overlay, AIR sparger, N2, O2, CO2) Software configured for cell culture applications 4-stage DO cascade BioPAT® MFCS software for data acquisition 1 integrated pump for pH control (base) Temperature control module for single-wall vessels 2 external signals 0 –10 V Standard test and documentation Installation set for the gas and water connections Power cable Single-wall culture vessel with tripod Heating blanket 120 | 230 V Stirrer shaft with single mechanical seal and direct coupling 1 addition bottle for correction agent Exhaust cooler Aeration filters 3-blade segment impeller Microsparger 4-way addition port Harvest pipe, height-adjustable Manual sampler Tool set for vessel dismantling Pt100 temperature sensor pH sensor DO sensor, amperometric
Options Control System Advanced DO controller Flexible switching CO2 from sparger to headspace Software for dual use MO | CC Mass flow controller Antifoam control via sensor Mechanical foam destroyer (MO applications) Level control via sensor Weight measurement substrates | culture vessel Gravimetric feed | level control Substrate addition via time profile Redox (ORP) measurement Turbidity measurement BioPAT® Xgas O2 | CO2 off-gas analysis System IQ | OQ BioPAT® MFCS SCADA system UniVessel® Glass Double-wall culture vessel Magnetic coupling for drive motor STT connectors for safe inoculation and media transfer Cooling finger (single-wall culture vessels) Spin filter for perfusion mode Aeration basket for organisms sensitive to shear stress Rushton impellers | 3-blade segment impellers Baffles Bottle holder Adapter for height reduction of exhaust cooler for the autoclave Inoculation port 3-way addition port Universal adapter ID 3.2 mm Harvest pipe bent for complete draining Port adapter 19 mm – 12 mm Set of consumables
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Longmead Longmead Longmead Business Longmead Business Business Centre Centre Business Centre Centre Longmead Business Centre Longmead Business Centre Unit 0800 1800 Vilvoorde Vilvoorde Vilvoorde 1800 Vilvoorde Unit Unit 5, 7-11 5, 5, 7-11 7-11 Unit Rodeo Rodeo Rodeo 5, Drive 7-11 Drive Drive Rodeo Drive 1800 1800Vilvoorde Vilvoorde Unit Unit5,5,7-11 7-11Rodeo RodeoDrive Drive Phone Blenheim Blenheim Blenheim Road, Blenheim Road, Road, Epsom Epsom Epsom Road, Epsom Phone +82.31.622.5700 Phone Phone +82.31.622.5700 +82.31.622.5700 +82.31.622.5700 Blenheim Road, Epsom Blenheim Road, Epsom Phone +82.31.622.5700 Phone +82.31.622.5700 Dandenong Dandenong Dandenong South Dandenong South South Vic Vic 3175 Vic South 3175 3175 Vic 3175 Dandenong DandenongSouth SouthVic Vic3175 3175FaxFax Surrey Surrey Surrey KT19 KT19 KT19 9Surrey QQ 9 9QQ QQ KT19 9 9QQ Fax +82.31.622.5799 ne hone Phone +32.2.756.06.80 +32.2.756.06.80 +32.2.756.06.80 Phone +82.31.622.5799 Fax+82.31.622.5799 +82.31.622.5799 Surrey KT19 Surrey KT19 9QQ QQ Phone +32.2.756.06.80 Phone+32.2.756.06.80 +32.2.756.06.80 Fax Fax+82.31.622.5799 +82.31.622.5799 +32.2.756.06.81 ax Fax+32.2.756.06.81 +32.2.756.06.81 Fax Phone Phone Phone +61.3.8762.1800 +61.3.8762.1800 +61.3.8762.1800 Phone +61.3.8762.1800 Fax +32.2.756.06.81 Fax+32.2.756.06.81 +32.2.756.06.81 Phone Phone Phone +44.1372.737159 +44.1372.737159 +44.1372.737159 Phone +44.1372.737159 Phone +61.3.8762.1800 Phone +61.3.8762.1800 Phone +44.1372.737159 Phone +44.1372.737159 FaxFax +61.3.8762.1828 Fax +61.3.8762.1828 +61.3.8762.1828 Fax +61.3.8762.1828 FaxFax +44.1372.726171 Fax+44.1372.726171 +44.1372.726171 Fax +44.1372.726171 Fax Fax+61.3.8762.1828 +61.3.8762.1828 Fax +44.1372.726171 Fax +44.1372.726171 gary Hungary ungary Hungary Hungary Hungary orius artorius Sartorius Stedim Stedim Sartorius Stedim Hungária Hungária Hungária Stedim Kft.Hungária Kft. Kft. Kft. Ukraine China China China China UkraineUkraine Sartorius Stedim Hungária Kft. Sartorius Stedim Hungária Kft. Ukraine China China Ukraine Ukraine yló agyló Kagyló u. 5u.u.Kagyló 55 u. 5 Sartorius Sartorius Sartorius Stedim Stedim Sartorius Stedim Biotech Biotech Biotech Stedim (Beijing) (Beijing) (Beijing) Biotech Co.Co. Ltd. (Beijing) Co.Ltd. Ltd. Co. Ltd. LLCLLC “Biohit” LLC“Biohit” “Biohit” LLC “Biohit” Kagyló Kagylóu.u.5 5 Sartorius SartoriusStedim StedimBiotech Biotech(Beijing) (Beijing)Co. Co.Ltd. Ltd. LLC “Biohit” LLC “Biohit” 2092 2092 Budakeszi Budakeszi Budakeszi 2092 Budakeszi No.No. 33 No.33 Yu’an 33Yu’an Yu’an No. Road Road 33 Road Yu’an Road Post Post Post Box Box 440 Box 440 Post “B” 440 “B” Box “B” 440 “B” 2092 2092Budakeszi Budakeszi No. 33 Yu’an Road No. 33 Yu’an Road Post Box 440 “B” Post Box 440 “B” Airport Airport Airport Industrial Industrial Industrial Airport Park Industrial Park Park Zone Zone Zone B Park B B Zone B 01001 01001 01001 Kiev, Kiev, Kiev, Ukraine 01001 Ukraine Ukraine Kiev, Ukraine ne hone Phone +36.23.457.227 +36.23.457.227 +36.23.457.227 Phone +36.23.457.227 Airport Industrial Park Zone Airport Industrial Park ZoneBB 01001 01001Kiev, Kiev,Ukraine Ukraine Shunyi Shunyi Shunyi District, District, District, Shunyi Beijing Beijing District, Beijing 101300 101300 101300 Beijing 101300 Phone +36.23.457.227 Phone +36.23.457.227 +36.23.457.147 ax Fax+36.23.457.147 +36.23.457.147 Fax +36.23.457.147 Shunyi ShunyiDistrict, District,Beijing Beijing101300 101300 Phone Phone Phone +380.44.411.4918 +380.44.411.4918 +380.44.411.4918 Phone +380.44.411.4918 Fax Fax+36.23.457.147 +36.23.457.147 Phone +380.44.411.4918 Phone +380.44.411.4918 Phone Phone Phone +86.10.80426516 +86.10.80426516 +86.10.80426516 Phone +86.10.80426516 FaxFax +380.50.623.3162 Fax+380.50.623.3162 +380.50.623.3162 Fax +380.50.623.3162 Phone Phone+86.10.80426516 +86.10.80426516 Fax Fax +86.10.80426580 Fax +86.10.80426580 +86.10.80426580 Fax +86.10.80426580 Fax +380.50.623.3162 Fax +380.50.623.3162 Italy aly Italy Fax Fax+86.10.80426580 +86.10.80426580 Italy Italy orius artorius Sartorius Stedim Stedim Sartorius Stedim Italy Italy Italy S.p.A. Stedim S.p.A. S.p.A. Italy S.p.A. Sartorius Stedim Italy Sartorius Stedim ItalyS.p.A. S.p.A. dell’Antella, ia Viadell’Antella, dell’Antella, Via 76/A dell’Antella, 76/A 76/A 76/A Sartorius Sartorius Sartorius Stedim Stedim Sartorius Stedim Biotech Biotech Biotech Stedim (Beijing) (Beijing) (Beijing) Biotech Co.Co. Ltd. (Beijing) Co.Ltd. Ltd. Co. Ltd. Via 76/A Viadell’Antella, dell’Antella, 76/A Sartorius Stedim Biotech Sartorius Stedim Biotech(Beijing) (Beijing)Co. Co.Ltd. Ltd. 12 0012 50012 Antella-Bagno Antella-Bagno Antella-Bagno 50012 Antella-Bagno a Ripoli a aRipoli Ripoli (FI) (FI) a(FI) Ripoli (FI) Shanghai Shanghai Shanghai Branch Shanghai Branch Branch Office Office Office Branch Office 50012 50012Antella-Bagno Antella-Bagnoa aRipoli Ripoli(FI) (FI) Shanghai Branch Shanghai Branch 3rd3rd Floor, 3rdFloor, Floor, North 3rd North North Wing, Floor, Wing, Wing, North Tower Tower Tower Wing, 1 1Office 1Office Tower 1 ne hone Phone +39.055.63.40.41 +39.055.63.40.41 +39.055.63.40.41 Phone +39.055.63.40.41 3rd Floor, North Wing, 3rd Floor, North Wing,Tower Tower1 1 No.No. 4560 No.4560 4560 Jin Jin No. Ke Jin Ke Road 4560 KeRoad Road Jin Ke Road Phone +39.055.63.40.41 Phone +39.055.63.40.41 +39.055.63.40.526 ax Fax+39.055.63.40.526 +39.055.63.40.526 Fax +39.055.63.40.526 No. Jin Road No.4560 4560 JinKe Ke Road 201210 Pudong Pudong Pudong District, District, Pudong District, Shanghai Shanghai District, Shanghai 201210 Shanghai 201210 201210 Fax Fax+39.055.63.40.526 +39.055.63.40.526 Pudong PudongDistrict, District,Shanghai Shanghai201210 201210 Phone Phone Phone +86.21.68782300 +86.21.68782300 +86.21.68782300 Phone +86.21.68782300 Phone +86.21.68782300 +86.21.68782332 |+86.21.68782300 68782882 FaxFax Fax+86.21.68782332 +86.21.68782332 FaxPhone +86.21.68782332 | 68782882 | 68782882 | 68782882 +86.21.68782332| 68782882 | 68782882 Fax Fax+86.21.68782332 Sartorius Sartorius Sartorius Stedim Stedim Sartorius Stedim Biotech Biotech Biotech Stedim (Beijing) (Beijing) (Beijing) Biotech Co.Co. Ltd. (Beijing) Co.Ltd. Ltd. Co. Ltd. Guangzhou Guangzhou Guangzhou Representative Guangzhou Representative Representative Office Office Office Office Sartorius Stedim Biotech (Beijing) SartoriusRepresentative Stedim Biotech (Beijing)Co. Co.Ltd. Ltd. Unit Unit Unit K, Building K,K,Building Building Unit 23 K,23 Building 23 23 Guangzhou Representative Guangzhou RepresentativeOffice Office Huihua Huihua Huihua Commerce Commerce Commerce Huihua &K, Commerce Trade &Building &Trade Trade Building Building & Building Trade Building Unit 2323 Unit K, Building No.No. 80 No.80 Xianlie 80Xianlie Xianlie No. Middle 80 Middle Middle Xianlie Road Road Road Middle Road Huihua Commerce &&Trade Huihua Commerce TradeBuilding Building Guangzhou Guangzhou Guangzhou 510070 Guangzhou 510070 510070 510070 No. No.8080Xianlie XianlieMiddle MiddleRoad Road Guangzhou Guangzhou 510070 Phone Phone Phone +86.20.37618687 +86.20.37618687 +86.20.37618687 Phone +86.20.37618687 | 37618651 |510070 37618651 | 37618651 | 37618651 FaxFax +86.20.37619051 Fax+86.20.37619051 +86.20.37619051 FaxPhone +86.20.37619051 Phone+86.20.37618687 +86.20.37618687| 37618651 | 37618651 Fax Fax+86.20.37619051 +86.20.37619051
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