Glucose 3q55

ccccccccccccccc cc c c c c c c c cccccccccccccccccccccccccccccccccc c c c c c c

c

|

| | !" |

c

# $|%&'&$ (

( # ( ) D-Glucose+O2ÿÿÿ * 2H2O2+Phenol+Aminophenazoneÿÿÿ aed quinone+H2O c | |% # &% +,,# & ( - # '

( #

# ! ./ ",, 0,,

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' 123 # |%&'&

45#%! Preparation of an extract of an orange

c

' + 6

0+ ( 7 8 +, 7 7,,,#

-,, 6

6 Dilution of orange extract

c

* 6

|%&'&

#5 '*

)

Dilution: ÷c

c

c

Orange extract(ml):

0.1

0.2

0.5

Distiled water(ml):

0.9

0.8

0.5

Dilution factor:

10

5

2

5 ½c Assay for glucose by the GOD-PAP method

0 c cc c

c

c

c

c

c

c

c

c

0c

c

c

c

c

c

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c

c

c

c

c

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c

ccc !"c #$c!c ! c%&'c

(c

)(c

*(c

+(c

,(c

#((c

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c

!c c %ù'c

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c- c %ù'c

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c c

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c#½(c$cc

c.!c!c $ c!/!c

' # -

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! 6 # -4 9$ 6 ( '

+

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# +,,

#

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c c

c

0 cc

c

c

c

c

c

c

c

c

c

c

c

cc

ccccccccccc c

c

c c

!c½c

c 0c

c (½(c

(½)c

(½*c

(½+c

(½,c

#½(c

!c% 'c

0!0 c c (½((c 1(($c

(½#2c

(½)2c

(½*c

(½1)c

(½++c

c

c

c

c

c

c

(½#2c

(½)3c

(½*c

(½12c

(½+,c

(½#*c

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c

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c

% !'c%$'cc

!c½c

c

45 (½#*c (½)3c (½3c

%4!'c%$'cc !c%0'c

0!0 c c (½((c 1(($c !c½c

! (½),c

! (½)6c (½3)c c

c

c

c

%4!'c%$'cc ! ! : ; -

Calculation of glucose content # * 4# 3 + 3 # -,, -,, -,,# 3 + -,, 3-,,

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ccccc c-c#((c%$'ccccc#,(ccc-ccc#((c ccc . c7!8c%$'ccc-ccc99999999999999ccc-ccc99999999ccc:#((cc c c

c

c

c

c

c

c

c

cccccc;cc c%'c

cccccccc#(+c


c % c #*"# "* $

# )

( ' *<= 6 # -, , 3 *= ; - 6) > > > > > >

6

*

! ) ' #

,, -+ * -+ # 6 6 |%&'& c c c c ;c"c#2+(c

-c

3